Journal: The American journal of pathology

Article Title: Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

doi: 10.1016/j.ajpath.2012.11.012

Figure Lengend Snippet: Figure 2 Outgrowth of growth plate and ectopic cartilage in perios- teum in tamoxifen-injected Col2CreER;b-catenin fl/fl mice. Col2CreER; b-catenin fl/flmice (CeJ) and their littermate b-cateninfl/flmice (A and B) received 100 mg/10 mL i.p. injections of tamoxifen per mouse at P5 to P7. Longitudinal sections of knee joints were prepared at 2 weeks (A, C, E, G, and I) or 5 weeks (B, D, F, H, and J) after completion of the tamoxifen injections. Arrows indicate out-growing growth plate (C) or ectopic cartilage masses embedded in the periosteum (D, H, and J). Boxed areas in C and D are enlarged in E and F, respectively.

Article Snippet: The American Journal of Pathology - ajp.amjpathol.org The mouse growth plate paraffin sections were incubated with the rabbit polyclonal antieb-catenin antibody (1:250; Cell Signaling Technology, Inc., Danvers, MA), followed by incubation with horseradish peroxidase conjugated antirabbit antibody (1:100; Vector Laboratories) and ImmPACT SG peroxidase substrate (Vector Laboratories) with methyl green counter staining.

Techniques: Injection

Journal: The American journal of pathology

Article Title: Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

doi: 10.1016/j.ajpath.2012.11.012

Figure Lengend Snippet: Figure 3 Localization of Cre-positive cells after tamoxifen injection in Col2CreER;b-catenin fl/fl;Rosa-LacZ mice. Col2CreER;b-catenin fl/fl;Rosa-LacZ mice (A, B, and DeF) and littermate b-catenin fl/fl;Rosa-LacZ mice (C) received 100 mg/10 mL i.p. injections of tamoxifen per mouse at P5 and P7. Longitudinal sections of knee joints were prepared at 1 (AeC), 2 (D and E), or 8 (F) weeks after completion of the tamoxifen injections and subjected to b-galactosidase staining. B and E: Magnified images of the boxed areas in A and D, respectively.

Article Snippet: The American Journal of Pathology - ajp.amjpathol.org The mouse growth plate paraffin sections were incubated with the rabbit polyclonal antieb-catenin antibody (1:250; Cell Signaling Technology, Inc., Danvers, MA), followed by incubation with horseradish peroxidase conjugated antirabbit antibody (1:100; Vector Laboratories) and ImmPACT SG peroxidase substrate (Vector Laboratories) with methyl green counter staining.

Techniques: Injection, Staining

Journal: The American journal of pathology

Article Title: Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

doi: 10.1016/j.ajpath.2012.11.012

Figure Lengend Snippet: Figure 4 Imbalance of cell proliferating activity in growth plate in the tamoxifen injected Col2CreER;b-catenin fl/flmice. Col2CreER;b-catenin fl/fl;Rosa- LacZ mice (A and B) and littermate b-cateninfl/fl;Rosa-LacZ mice (C and D) received 100 mg/10 mL i.p. injections of tamoxifen per mouse at P5 and P7. Longitudinal sections of knee joints were prepared at 2 weeks after completion of the tamoxifen injections and subjected to PCNA staining. E: Ratios of PCNA- positive cells were measured (see Materials and Methods). *P < 0.05

Article Snippet: The American Journal of Pathology - ajp.amjpathol.org The mouse growth plate paraffin sections were incubated with the rabbit polyclonal antieb-catenin antibody (1:250; Cell Signaling Technology, Inc., Danvers, MA), followed by incubation with horseradish peroxidase conjugated antirabbit antibody (1:100; Vector Laboratories) and ImmPACT SG peroxidase substrate (Vector Laboratories) with methyl green counter staining.

Techniques: Activity Assay, Injection, Staining

Journal: The American journal of pathology

Article Title: Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

doi: 10.1016/j.ajpath.2012.11.012

Figure Lengend Snippet: Figure 5 PCNA, collagen 10, TUNEL, and TRAP staining in ectopic cartilaginous masses at early and late stages after tamoxifen injections. Col2CreER;b-catenin fl/fl;Rosa-LacZ mice received 100 mg/10 mL i.p. injec- tions of tamoxifen per mouse at P5 and P7. Longitudinal sections of knee joints were prepared at 2 weeks (A, C, E, G, and I) or 8 weeks (B, D, F, H, and J) after completion of the tamoxifen injections and subjected to PCNA (A and B), collagen 10 immunostaining (C and D), TUNEL (E and F), and TRAP (I and J) staining. The arrows in E indicates apoptotic cells. G and H: Phase contrast images (E and F), respectively.

Article Snippet: The American Journal of Pathology - ajp.amjpathol.org The mouse growth plate paraffin sections were incubated with the rabbit polyclonal antieb-catenin antibody (1:250; Cell Signaling Technology, Inc., Danvers, MA), followed by incubation with horseradish peroxidase conjugated antirabbit antibody (1:100; Vector Laboratories) and ImmPACT SG peroxidase substrate (Vector Laboratories) with methyl green counter staining.

Techniques: TUNEL Assay, Staining, Immunostaining

Journal: The American journal of pathology

Article Title: Loss of β-catenin induces multifocal periosteal chondroma-like masses in mice.

doi: 10.1016/j.ajpath.2012.11.012

Figure Lengend Snippet: Figure 8 Immunolocalization of b-catenin in ostechondromas and rib growth plates. AeL: Sections of osteochondromas from HME patients (A, B, E, F, I, and J) and rib growth plate obtained from autopsy (C, D, G, H, K, and L) were subjected to immunofluorescence staining for b-catenin. Cartilage caps of osteo- chondromas were divided into two parts: a region composing of solitary and relatively small cells (Solitary), and a region adjacent to the bone and composed of relatively large cells (Large). Resting zone (Resting) and hypertrophic zone (Hypertrophy) of growth plates were separately inspected. AeD: b-catenin staining; (EeH) merged images of b-catenin staining (red) and DAPI nuclear staining (blue); (IeL) phase contrast images (AeD), respectively. M: Ratios of b-catenin positive cells were evaluated as previously described (see Materials and Methods). *P < 0.05.

Article Snippet: The American Journal of Pathology - ajp.amjpathol.org The mouse growth plate paraffin sections were incubated with the rabbit polyclonal antieb-catenin antibody (1:250; Cell Signaling Technology, Inc., Danvers, MA), followed by incubation with horseradish peroxidase conjugated antirabbit antibody (1:100; Vector Laboratories) and ImmPACT SG peroxidase substrate (Vector Laboratories) with methyl green counter staining.

Techniques: Staining